Development of an Efficient Fermentation Process by Response Surface Methodology for Enhanced Dextransucrase Production from Leuconostoc lactis KU665298

Abstract

Dextransucrase is an important extracellular glucosyltransferase which catalyzes the synthesis of dextran from sucrose and valuable oligosachharides. Dextran is a high molecular weight glucose polymer, which has diverse applications in the area of medicine, food and fine chemical industries. A CCD based statistical approach was employed for the enhanced dextransucrase production by Leuconostoc lactis KU665298, isolated from sugarcane mill bagasse sample. The most determining factors (i.e. incubation time, inoculum size, sucrose and K2 HPO4 ) for dextransucrase production by L. lactis were screened by Plackett Burman design. Maximum dextransucrase activity of 3.6 U/mL was obtained with the experimental conditions of incubation time 18h with inoculum volume 6.5 (%, v/v) in a medium (pH 8), containing (%, w/v) sucrose 2, K2 HPO4 2.5, which corresponded well with the predicted value of 3.05 U/mL by the model.