Analysis of Physicochemical Variables and Their Interactive Effect on Uricase Production from Alcaligenes faecalis

Abstract

Uricase catalyzes the conversion of uric acid into allantoin and hydrogen peroxide by oxidation reaction. It has therapeutic potential in treating gout, hyperuricemia and analysis of uric acid in biological fluids. The interactive effect of various physico-chemical variables on uricase production from isolated Alcaligenes faecalis strain has been studied through response surface methodology. The role of most significant factors were evaluated and medium pH (7.0), inducer (uric acid) concentration (3 g/L), agitation rate (150 rpm) and incubation time (45 h) were found to play critical role in the production of enzyme. The production of uricase by A. faecalis increased up to 2.5-fold after applying the RSM.