Bioactive Streptomyces sp. S5 Associated with Marsilea minuta Linn.; Purification and Characterization of Antimicrobial Substance

Abstract

Endophytic actinomycetes are the hidden treasure of future pharmaceutical research. Plants with ethno medicinal value are most important resource of endophytic microorganisms possessing bioactivity. Marsilea minuta Linn., a very common pteridophyta and is well known for being used as folk medicine. In this investigation we have isolated 7 actinomycetes strains form suface sterilized petioles of this plant. Antimicrobial property evaluation of these isolates revealed one strain, S5 with both antibacterial and antifungal property. The strain, S5 was identified as Streptomyces sp. S5 through polyphasic approach. Active antimicrobial substance was isolated from S5 fermented cell free liquor and purified by activity guided TLC and analytical HPLC study. Purified substance was found to inhibit bacterial pathogen; Aeremonas caviae, Bacillus cereus and plant pathogenic fungi Fusarium solani, Sclerotinia sp. UV and FTIR spectrum of purified substance indicated presence of phenolics with poly-ene aliphatics as active principal. However further characterizations are necessary for structural information of the antimicrobial substance.