Characterization and Identification of Biofilm Forming Bacterial Isolate Shewanella sp. DDR4

Abstract

Bacteria can adhere to natural or artificial surfaces and form sessile multicellular communities known as biofilms. The natural and artificial surfaces covered by biofilms include cells and tissues of organisms, soils, sediments, pore in glaciers, thermal vent, pipelines, heat exchangers, separation membranes, and filters. In the marine environment, biofilms cover most subtidal and intertidal solid surfaces such as rocks, ships, loops, marine animals, and algae. Totally 10 bacterial isolates were obtained from three different ships anchored at the Royapuram harbour, Chennai, Tamil Nadu, India and screened for biofilm forming activity. The bacterial isolate DDR4 showed biofilm forming activity in the microtiter plate assay with a significant optical density of 0.632. Also an attempt was made to characterize the morphological, biochemical and molecular properties. Partial sequences of the 16S rRNA genes of the marine bacterial isolate was determined following the amplification of 16S rRNA genes, these sequences were aligned with the sequences of representative species of the genus Shewanella sp. and phylogenetic characters of the isolate Shewanella sp EF559251 with other closely related bacterial isolates were analyzed.