Evaluation of Disinfectants on Growth of Bacteria Associated with Pinning Stage of Paddy Straw Mushroom Cultivation

Abstract

Pinning stage is extremely crucial for mushroom cultivation, many bacteria infect mushroom in this stage due to its proteinaceous nature. In the present study, eight bacteria were isolated from the pinning stage of paddy straw mushroom Volvariella volvaceae. The bacterial isolates were tentatively identified by morphological, physiological and standard biochemical tests. Out of the eight bacteria were isolated, Staphylococcus saprophyticus, Bacillus pumilis, Bacillus sp. and Kingella sp. were inhibiting growth of Volvariella volvaceae. These contaminating bacterial isolates showed resistance to most of the antibiotics used. Then, the bacterial isolates were subjected to various disinfectants such as calcium hypochlorite, hydrogen peroxide and calcium hydroxide added at different concentration. It was observed that, 300 ppm of calcium hydroxide solution is economic and reduces growth of contaminating bacterial isolates. Thus, concluded field study and nutritional quality analysis mushroom is essential to use calcium hydroxide as a cost effective control measures to increase paddy straw mushroom production.