Synthesis and Comparative Characterization of Fungal AgNPs from Isolated Colletotrichum ti and Paecilomyces sinensis: Endophytes of Oroxylum indicum (L.) Vent.

Abstract

The study emphasizes on the use of a biological approach for the synthesis of silver nanoparticles (AgNPs). The fungal endophytes have been of great importance for such an approach. An experiment was designed to employ Colletotrichum ti & Paecilomyces sinensis: fungal endophytes of Oroxylum indicum (L.) Vent. (an important medicinal tree) for green synthesis of AgNPs which are well characterized by the implementation of fluorescence microscopy, UV-visible spectroscopy, fourier transform infrared spectroscopy and X-ray diffraction techniques. The fluorescence microscopy helps to define cell physiology more precisely whereas UV-visible spectroscopy shows a characteristic peak of silver nanoparticles at 420 nm. FTIR graph implies that with increasing concentrations of silver nitrate in fungal samples, the association is stronger which is reflected by the strong vibrations in the similar regions of wavelength. The size of the synthesized nanoparticles was determined by XRD analysis. The calculated crystalline size of the intracellular AgNPs is 14.69 nm. A comparative study was developed by synthesizing extracellular and intracellular nanoparticles with Paecilomyces and Colletotrichum respectively.