Variations of Microbial Community Composition in Turfgrass Soil at Different Depth Using Illumine Sequencing

Abstract

Many studies have been carried out to assess the effects of plant on soil microorganism communities in different environments. However, little is known about whether soil depths affect soil microbial community in turfgrass ecosystems. Therefore, in the present study the microbial diversity in turf-grass soil at different depth was studied by high-throughput sequencing technique, and the corresponding analysis of microbial composition were conducted. The results showed that the bacteria and fungi in the turf soil at depths of 0-10 cm were richer than that of 10-20 cm. The difference of soil microbial community at the genera level is more significant than that at phylum level. Among bacteria, a total of 3 dominant genus and 148 nondominant genera were shared by the two samples. Two dominant genera and 12 non-dominant genera were present only in sample CP3 (10-20 cm), while 2 dominant genera and 18 non-dominant genera present only in sample CP4 (0-10 cm). Among fungi, a total of 7 dominant genera and 79 non-dominant genera were shared by the two samples. Ten non-dominant genera were present only in sample CP3, while 6 dominant genera and 21 non-dominant genera present only in sample CP4. The results also indicated that both dominant and nondominant microbial populations differed greatly in the two samples, as did the overall soil microbial community structure. This study provides previously unknown information regarding the impact of soil depths on microbial communities in turfgrass soil and also lays a foundation for further investigations into microbiota in turfgrass ecosystems.