Probiotic Characterization and Quantification of Folates Produced by Bacillus Strains Isolated from Infant Faecal Matter

Authors

  • Smita Hasini Panda Department of Microbiology, North Orissa University, Baripada, Odisha-757003, India Author
  • Jyothsna Khanna Goli Department of Microbiology, Osmania University, Hyderabad- 500007, Andhra Pradesh, India Author
  • Sushrirekha Das Department of Microbiology, North Orissa University, Baripada, Odisha-757003, India Author
  • Nakulananda Mohanty Department of Microbiology, North Orissa University, Baripada, Odisha-757003, India Author

DOI:

https://doi.org/10.5530/jam.1.1.3

Keywords:

Probiotic, lactic acid bacteria, Bacillus, folic acid, infant faecal matter, microbiota, gut

Abstract

The aim of the present study was to characterize the probiotic qualities and to study the efficacy of the selected isolates to produce folic acid by microbiological assay. Three isolates, IFM22B, IFM24B and IFM25B were selected for folate production in folic acid casei medium and the net folate yield was 59 ng/ ml, 56 ng/ml and 49 ng/ml, respectively. The three isolates showed more than 98 % similarity to Bacillus spp. by 16S rRNA sequencing. Spores of these strains showed excellent tolerance in partially simulated gastrointestinal tract conditions and exhibited antimicrobial activity against organisms such as Staphylococcus aureus, Klebsiella and Escherichia coli. Importantly, these isolates were susceptible to the most of the antibiotics tested, in conflict that they would not donate resistance determinants if administered in the form of probiotic preparations.

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Published

2024-01-31

How to Cite

Probiotic Characterization and Quantification of Folates Produced by Bacillus Strains Isolated from Infant Faecal Matter. (2024). Journal of Advanced Microbiology, 1(1), 23-27. https://doi.org/10.5530/jam.1.1.3

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