Superoxide Dismutase and Oxidative Stress: Elucidation of Antiaging and Antioxidative Effect on Mammalian Cell Lines

Abstract

During the course of routine metabolic activities of a cell, it produces different harmful byproducts esp. reactive oxygen species. Superoxide dismutase (SOD; EC 1.15.1.1) is a cellular defense enzyme that neutralizes these free radicals and protects the cell from deleterious effects and increases longevity. In the present work, superoxide dismutase enzyme, purified from a thermophilic bacterium Bacillus licheniformis SPB13, was assessed for its antioxidative effect on HeLa cell lines. An experimental design was constructed in which the animal cells were first of all exposed to an oxidative stress generated using chemical stressors like H2
O2 andMenadione, a vitamin K analogue. Then the MTT assay was performed to assess the cell viability in the presence of high oxidative stress. In final experiment, the menadione preincubated HeLa cells were treated with purified SOD and kept for an interval for detoxification of ROS. Viability assay was performed and it showed an increase in cell viability by 65 % at a concentration of 30 μg/ml of SOD. This confirmed the antioxidative and antiaging property of thermostable SOD from Bacillus licheniformis SPB-13. This potential can be harvested by pharmaceutical industry for topical antiaging formulations.