Enzymatic Hydrolysis of Mangifera indica Saw Dust and its Use as Potential Feedstock for the Citric Acid production and Single Cell Rotein

Abstract

Enzymatic hydrolysis of cellulose obtained from Mangifera indica saw dust after high pressure steaming and alkali treatment, was carried out by the partially purified cellulase and β-glucosidase obtained from Trichoderma viride and Aspergillus wentii, respectively. The incubation period 48 h, temperature 50 0 C and 30 units (IU) of cellulase per gram of cellulose fiber were sufficient for 67 % Saccharification at pH, 4.8. Below and above pH-4.8 the percent hydrolysis of cellulose declined sharply. Supplementation of Aspergillus wentii, β-glucosidase to Trichoderma viride cellulase, nearly 80 % of cellulose was hydrolyzed in 48 h compared to 54.6 % without β-glucosidase under optimum condition. The enzymatic hydrolysate was further used for the production of yeast biomass and citric acid fermentation. Mangifera indica residue which can be used as a substrate for enzyme production which reduces the cost of enzyme production and enzymatic conversion of carbohydrate part of Mangifera indica into fermentable sugar.