Production of Collagenase from a Novel Non-pathogenic Isolate Chryseobacterium contaminans KU665299

Abstract

Collagenase is a vital therapeutic enzyme that carries out the degradation of collagen, the most abundant protein in vertebrates, into small peptide fragments and has potential applications in medical and meat based food industries. In the present study, isolation and screening of collagenase producing bacteria from soil samples were executed. A novel and non-pathogenic bacterial isolate, identified as Chryseobacterium contaminans by 16S- rRNA sequence analysis, with collagenase activity was reported. The sequence was deposited in GenBank with accession number KU665299. This is the pioneer report on the production of collagenase by Chryseobacterium sp. Maximum extracellular collagenase production (2.0 U/mL) by obtained when this isolate was grown in medium (pH 6.5) containing (%, w/v) sucrose 1.0, peptone 1.0, gelatin 0.3, yeast extract 0.2 and few salts for buffering action at incubation temperature 30o C and agitation 150 rpm for 24 h of fermentation.