Detection of Exogenous and Endogenous (Cell Culture Based Analysis) Production of Cellulase in Grass Carp (Ctenopharyngodon idella)

Abstract

Cellulase activity was found in forty eight bacteria isoalted from the grass carp intestine and intestinal content samples. Twenty six bacteria were positive towards congo red reaction. β-glucosidase and exoglucanase activity was measured in bacterial cultures but endoglucanase activity could not be detected. This paper reports the first attempt of using intestinal and hepatopancreatic cell culture for the establishment of the endogenous production of cellulase in Ctenopharyngodon idella (grass carp). EMEM media was used for cell culture. Initial experiment for primary cell culture by trypsinization showed no enzyme (β-glucosidase) production but enzyme was detected in explant culture samples. An increase (80 % of intestinal and 66 % of hepatopancreatic samples) in enzyme activity was seen when CMC was used as inducer