Journal of Advanced Microbiology
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Abstract: A thermophilic Streptomyces sp. producing an extracellular alkaliphilic lipase was selected
after screening of 30 bacterial isolates obtained from a water sample of a hot-spring. The Streptomyces sp.
designated STL-D8 produced a maximum zone of hydrolysis on the tributyrin (0.5 % v/v) agar plate during
primary screening. This bacterial isolate produced 16 U/ml of lipase in the broth containing DMSO (5 % v/v) at
55oC and pH 8 in 48 h. Supplementation of production broth with cottonseed oil (2 %, v/v) enhanced the lipase
activity to 17.8 U/ml. Carbon and nitrogen sources were optimized and a mild increase in lipase activity (18.9 U/
ml) was achieved with glucose (0.4 % v/v) added to the production broth. The lipase production by Streptomyces
sp. further increased in the presence of yeast extract (0.4 % w/v; 19.6 U/ml) and NaNO3 (0.4 % w/v 24.4 U/ml).
Thus a production broth containing cottonseed oil, glucose, yeast extract and NaNO3 was used to optimally
produce extracellular lipase by Streptomyces sp. in the presence of selected solvents (dimethyl sulfoxide
(DMSO), diethylphathalate (DEP), toluene, xylene, n-pentane, n-hexane, n-heptane, methanol, ethanol, npropanol,
isobutanol and n-octanol) added to the broth at 10-40 % (v/v) concentrations. The lipase production
was least affected by DMSO, n-pentane and n-hexane (40 % v/v). The lipase production occurred frequently at
higher solvent concentration and both the hydrophobic nature (Log P value) as well as the nature of groups in
the organic solvent(s) appear to affect the cell growth and lipase production by Streptomyces sp.