Abstract: A callus induction and plant regeneration protocol was developed from leaf explants of the Psoralea corylifolia Linn. Explants were cultured on MS medium supplemented with different plant growth regulators (PGRs) i.e. 2, 4-D, BAP and KN. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearance of callus and the potential of callus differentiation. 2, 4-D (1.0 mg/l) alone and 1.0 mg/l of 2, 4-D with 0.5 mg/l KN have been found highly effective in callusing with quite good in texture and friable in nature i.e. 65.5 % to 75.0 % respectively. After the callus formation the induced callus were sectioned into small pieces and transferred on MS media supplemented with BAP and KN separately or BAP and KN with NAA, IBA and IAA. MS medium containing 1.5 mg/l BAP with 0.5 mg/l NAA was found to be the best medium for maximum in vitro response i.e. (80.0 %) shooting and multiple shoots (6.0) with maximum 7.5 cm in length. Best rooting was achieved on full strength MS medium fortified with 1.0 mg/l IBA respectively.