Journal of Advanced Microbiology

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Abstract: Diarrhoea has a high impact on the rate of morbidity and mortality of infants less than five

years in developing countries. Among different etiological agents, the most common causal organisms for

persistent diarrhoea in children are enteric bacterial genera like Escherichia, Shigella, Salmonella, Klebsiella

and Enterobacter. Out of them E. coli is the most dominant and have several pathotypes that are commonly

referred as enteropathogenic E. coli (EPEC). Apart from the species E. coli, Escherichia albertii KF1 has

recently been identified as an emerging enteropathogen associated with infant diarrhoea. In this study we have

performed in silico analyses on the genome sequence of this strain using different bioinformatic platforms and

tried to identify regions or genes related to virulence which are in common to the prototypical Escherichia coli

O127:H6 E2348/69 and uniquely present in it. Both the genomes share 3374 CDS, with some conserved

pathogenicity encoded features like the typical enteropathogenic type three secretion system (T3SS) associated

with locus of enterocyte effacement (LEE) and non-LEE effectors. In addition, several singletons are also

identified in the genome of E. albertii KF1 within eight potential genomic islands (GEIs). This includes genes

encoding barrel domain-containing outer membrane with a putative type five secretion system (T5SS), multidrug

export proteins (MATE) and a region coding for flagellar synthesis and a large number of transposases and

hypothetical proteins of unknown functions. Therefore, the study is very relevant to the complete understanding

of the importance of exclusive GEIs and virulence related singletons along with the conserved coding regions

to determine the pathogenicity and emergence of E. albertii KF1 as an enteropathogen.