Abstract: Transition from an opportunistic pathogen to a deadly disease forming one, depends on a
number of aspects, which activate causative protein production and their functions. In recent world, most of
the proteins are still functionally unknown i.e. hypothetical proteins (HPs) and are not negligible. In the
present study, functional categorization and structure prediction of 20 HPs of Candida glabrata was performed.
Their physicochemical properties, functional domains, as well as interacted proteins were predicted and
analyzed. Signal peptide, subcellular localizers, transmembrane regions were determined to explore physical
characteristics of selected HPs. They were involved in various important functions such as nuclear-vacuolar
junction, metabolic pathways, ATPase execution, DNA binding, mitochondrial transportation, amino acid
biosynthesis and catabolism, vesicular transportation, extracellular, DNA repair and cell cycle control, nuclear
functions, mitochondrial RNA synthesis and translation, RNA synthesis, glucose transportation and Ca2+ ion
exchanger. 3D structure of each HP was determined and energy minimization was done by using GROMOS96
force field implicated in Swiss-Pdb Viewer. Ligand binding sites of the HPs showed the active regions,
involved in functional modulation. Thus in silico analysis of HPs is more easy to reveal the structures and
functions, which was experimentally very expensive and tedious. It would also be helpful in recognizing the
mechanism of pathogenesis and in developing therapeutic drug molecules and their docking studies.